Isotope elution profiles

Elution Profile. Elution profiles are determined with the in-line radiation detector. For calibration of the in-line detector, equations developed for quantitative radiochromatography may be adapted. For a single detector, isotope, and geometry, which is in effect controlled by the tubing used to carry the eluate past the detector, a simple expression can be written (Eq. 2) ... 

Figure 2.206 Chromatographic elution profiles of COj with natural abundance resulting in the typical S-shaped curvature when displaying the calculated 5-values, (a) note the intensity difference of the isotopes...

The identity of the eluted compounds is indicated at the top of the peciks. With the exception of the GR HX and XMP ATP pairs, other components in this profile were well separated. The peaks illustrated by the UV profile represented approximately 75 nmoles of each of the 16 standards. The peaks documented by the isotope counter represented 80-100 picomoles of radioactive standards. In this sample, radioactive X, XR and GMP were left out. Small amounts of radioactive impurities which were present in some of the standards, especially guanine, and eluted in an early peak. 

Uranium concentration profile and isotope separation factors for a U(VI)-U(IV) sample eluted from an ion-exchange column see text (Nomura et al. 1996)... 

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