Isoflurane detection

Fig. 2. Simultaneous detection of multiple fluorinated molecules in vivo. To explore the hypothesis that uptake of the anticancer drug 5FU by tumors is pH dependant, we infused 5FU (0.4 ml (50 mg/ml) IV), the extracellular pH reporter CF3POL (400 mg/kg IP), and the chemical shift standard NaTFA (200 mg/kg IP) into an anesthetized rat (1% isoflurane) with a subcutaneous 13762NF breast tumor (1.4 x 1.5 x 1.1 cm). Thirty minutes after administration, all four molecules were detectable simultaneously in 17 min. At this stage, no metabolites of 5FU were detected.

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