Isoelectric focusing rehydratable gels

Rehydratable Polyacrylamide Gels for Ultrathin-Layer Isoelectric Focusing... [Pg.54]

For many years rehydratable granulated dextran (Sephadex) or polyacrylamide (Bio-Gel) gels have been used for gel chromatography and in the form of hydrated gel layers for analytical and preparative isoelectric focusing (l t, 15). Recently, rehydratable layers of granulated gels on a plastic film have been developed ( ). The presence of carrier ampholytes or addition of 1-2 % glycerol was necessary... [Pg.55]

Table III. Electrical Conditions on Isoelectric Focusing in Rehydratable Gels (60-120 m).

Figure 3. The effect of separators on ultrathin-layer isoelectric focusing in rehydratable gels. Gels (120 jjim) containing 10% Dextran 35 plus 3% glycerol were rehydrated with 3% Servalyt pH 4-6 plus 5%, glycerol with the indicated concentration of different separators. On the left, a mixture of four marker proteins MYH-horse myoglobin, CAR-carbonic anhydrase, LAC-(3-lactoglobulin,...

Standardization in Isoelectric Focusing on Ultrathin-Layer Rehydratable Polyacrylamide Gels... [Pg.117]

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