Introducing the small biomolecules

Peptides, coenzymes, amino acids are special functional biological molecules. They became a hot topic while studying the analogue enzymes. For example, the ALT model was formed by connecting the cofactor pyridoxamine phosphate (pyridoxamine, vitamin Be amine) and ethylenediamine to y3-CD. The two functional groups were located through the intermediate positioning process. 

Tripeptide Ser-His-Asp had been introduced to the CD for simulating the chymotrypsin by the following method. Mix 2-amino ethyl mercaptan salts, 6-0-tosyl-/3-CD and NH4HCO3 with DMF/H2O (1 3) solution under nitrogen protection at 60 C for 4h. After vacuum concentration, separate the product by Sephadex G-10 column. The yield is about 43%. Mix the above product with Boc-Ser (Ot-Bu) -His-Asp (Ot-Bu) OH, dicyclohexyl carbodiimide, 1-hydroxy triazole in DMF solution to obtain 6-[Boc-Ser (Ot-Bu) -His-Asp (Ot-Bu) NHCH2CH2S]-/3-CD. Then treat with the TFA/CH2C12(9 1) mixed solution, vacuum concentrate and freeze-dry. This modified CD can be used as an analogue enzyme to hydrolyze nitrophenol esters. 

In addition, some researchers have tried to introduce the enzyme nicotinamide drug molecules into the CD skeleton to deliver the drug to specific locations. 

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