Interphase chromatin

Rattner JB, Hamkalo BA (1979) Nucleosome packing in interphase chromatin. J Cell Biol 81 ... 

Abstract In eukaryotic cells, relaxed interphase chromatin undergoes pronounced changes... 

Interphase chromatin must be much less tightly... 

Nucleosomes are connected to one another by linker DNA of variable length and the linker-binding histone HI protein (Fig. 1) (7). These long arrays of nucleosomes spontaneously condense to form helical arrays of nucleosomes, termed the 30-nm fiber after its apparent diameter (Fig. 1) (8). Additional condensation and compaction of chromatin occur through intemucleosomal interactions. One important internucleosomal interaction required for chromatin fiber formation is the interaction of a highly acidic patch of histone H2A with the histone H4 tail (8). Ultimately, these internucleosomal interactions form interphase chromatin with an unknown architecture (Fig. 1) (9). 

Belmont, A. S., Braunfeld, M. B., Sedat, J. W., and Agard, D. A. (1989). Large scale chromatin structural domains within mitotic and interphase chromatin in vitro and in vivo. Chromosoma 98, 129-143. 

Fig. 42. Interphase chromatin fibers as seen at higher magnification (61, 900 X). Thickenings are visible on the fibers which in some places (arrow) have helical twists (Du Praw, 1965a).

Fig. 46, Folded fiber model of the chromosome (Du Praw, 1965a, 1965b, 1966). a) Each interphase chromatin fiber consists of a DNA molecule held in the form of a secondary helix by means of its protein envelope b) replication of the fiber takes place successively from both ends toward the middle c) after replication the daughter fibers are folded into a condensed, compact chromosome. It is postulated that this folding is brought about by contractile protein molecules in the membrane d) scheme of chromatid stracture.

Pellicciari, C. Marchese, G. Bottone, M. G. Manfredi Romanini, M. G. Kinetics of DNase I digestion of interphase chromatin in differentiated cell nuclei of the mouse a flow cytometric study. Basic Appl. Histochem. 1987,31, 501-509. 

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