Interleukin three-dimensional structure

Clore GM, Appella E, Yamada M, Matsushima K, Gronenbom AM. Three-dimensional structure of interleukin 8 in solution. Biochemistry 1990 29 1689-96. 

Figure 2.8 Three-dimensional structure of (a) human interleukin-4, as determined by NMR, and (b) human follicle-stimulating hormone, as determined by X-ray diffraction. Reproduced from protein data bank (www. rcsb.org/pdb, molecule ID numbers 1ITM and 1 FL7 respectively)...

F. Three-Dimensional Structure of Interleukin-1 Beta Con verting Enzyme (ICE)... 

Fig. 1. Comparison of the three-dimensional structures of human Interleukin-8 (green) MCP-1 (blue) and Fractalkine (EST Z44443) (red). The 11-8 structure is taken from the Protein Database (PDB) entry (1IL8), and the MCP-1 structure is a model built of the NMR structure of MI P-l (> (PDB entry 1HUM). The intrachain disulfide bonds are shown in yellow. The model for the chemokine domain of Fractalkine was built using the SwissModel server (16,17). As can be seen the three structures show a large degree of conservation of the overall structure, despite a relatively low level of primary sequence identity. The additional three amino acids in Fractalkine are accommodated as a 310 helix between the two N-terminal cysteines. The steric requirements here presumably forbid a CX2C motif. The model building software can be accessed at http www.expasy.ch swissmod SWISS-MODEL.html...

G. M. Clore, P. T. Wingfield, and A. M. Gronenborn, Biochemistry, 30,2315 (1991). High-Resolution Three-Dimensional Structure of Interleukin IB in Solution by Three- and Four-Dimensional Nuclear Magnetic Resonance Spectroscopy. 

The three-dimensional structures are known for many gplSO cytokines including murine LIF (Robinson et al, 1994), human LIF (Hinds et al, 1998), CNTF (McDonald et al, 1995), human Interleukin 6 (IL-6) (Somers et al, 1997), HHV-8 IL-6 (Chow et al, 2001a) and oncostatin M (OSM)... 

R. T. Kroemer, S. W. Doughty, A. J. Robinson, W. G. Richards. Prediction of the three-dimensional structure of human interleukin-7 by homology modeling. Protein Eng. 

Brandhuber BJ, Boone T, Kenney WC, McKay DB (1987) Three-dimensional structure of interleukin-2. Science 238 1707-1709... 

R. Sarabu, K. Lovey, V. S. Madison, D. C. Fry, D. N. Greeley, C. M. Cook, and G. L. Olson, Tetrahedron, 49, 3629 (1993). Design, Synthesis and Three-Dimensional Structural Characterization of a Constrained H-Loop Excised from Interleukin la. 

Feng Y., Klein B.K., and McWherter C.A. (1996), Three-dimensional solution structure and backbone dynamics of a variant of human interleukin-3,./. Mol. Biol. 259, 524-541. 

Powers, R., Garrett, D.S., March, C.l. et al. (1992). Three-dimensional solution structure of human interleukin-4 by multidimensional heteronuclear magnetic resonance spectroscopy. Science 256, 1673. 

Three members of the interleukin-1 family have been cloned (Dinarello 1996). All bind both types of receptors but only IL-la and IL-ip (encoded on chromosome 2) are agonists. The third is the interleukin 1 receptor antagonist protein (IRAP). All share 20-25 % amino acid homology. Mature IL-la and IL-ip have similar three-dimensional open barrel structures of P sheets. 

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