Interaction with biotinylated liposomes

Fig. 2. A Interaction of biotin with the tetrameric protein streptavidin. B Schematic representation of the aggregation of biotinylated liposomes induced by the tetrameric streptavidin protein (taken from [16])...

Figure 15 (A) Faradaic impedance spectra of (a) The (17)-functionalized An electrode, (b) After interaction of the sensing electrode with (18) (5 X 10" M), which was pretreated with (20) (1 X 10 M, 30 min, 25°C). (c) After treatment of the resulting electrode with avidin (2.5 pg-mL" ). (d) After interaction with the biotinylated liposomes, (21). (e) After treatment of the interface for a second time with avidin (2.5 0,g mL" ), (f) After interaction of the interface for a second time with the biotinylated liposomes, (21). Data were recorded in 0.1 M phosphate buffer, pH = 7.2, in the presence of [Fe(CN)g] " , (1 1), as redox-probe. (B) Calibration curve corresponding to the changes in the electron transfer resistances of the sensing electrode upon interaction with the analyte DNA, (18), at different concentrations and enhancement of the sensing process by a double-step avidin/ biotinylated liposome amplification path. corresponds to the difference in the electron transfer resistance after a double-step avidin/biotinylated liposome amplification and the electron resistance of the (17)-functionalized electrode.

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