Intensity sensing

Keywords Anisotropy Intensity sensing Time-resolved fluorimetry Wavelength ratiometry... 

Using the long-lifetime emission as a reference in intensity sensing by shortlifetime dye. This approach known as dual luminophore referencing (DLR) will be considered in the next section. 

In intensity sensing, the most efficient and commonly used method of intrinsic referencing is the introduction of a reference dye into a sensor molecule (or into support layer, the same nanoparticle, etc.) so that it can be excited together with the reporter dye and provide the reference signal [1], The reference dye should conform to stringent requirements ... 

Fig. 3 Intensity sensing (a) and this sensing with the reference dye (b). The fluorescence intensity with the band maximum at decreases as a function of analyte concentration. The reference dye allows providing the ratio of two intensities detected at wavelengths ki and X2...

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As the particles randomly diffuse through the solution, the location of the dark rays of constructive interference will change therefore the intensity sensed by the fixed detector will vary, i.e. the interference pattern produced by the scattering particles will be modulated by the particle motions. The intensity fluctuations at the detector, though random, will be more rapid for small, rapidly moving particles than for... 

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