Inoculation and maintenance of culture system

Resuspend inoculum (5.0 x 10 cells bed volume) in 800 ml (bed void volume) fresh medium. 

Gently swirl the inoculation vessel to ensure an even cell suspension. 

Slowly pump the inoculum into the bed with air pressure (hand pump), being careful not to introduce air bubbles into the system. 

For suspension cells, start perfusing immediately at 40 ml mim or at a linear flow velocity of 2 cm murk 

For anchorage-dependent cells, leave stationary for 2-4 h for the cells to attach before perfusing at 40 ml min k 

---