Implants cellular-based approaches

Sewell et at s method or its modification (Sewell et al, 1955).The enzyme his-tochemical approach is a more objective, quantitative, consistent and reproducible method than cellular response, but requires more time, resources and expertise. Enzyme histochemistry is based on the fact that any cellular response to a foreign material is always associated with the presence of a variety of enzymes. The enzymatic activity of a suture implant site is quantified by microscopic photometry of a cryostat section of the tissue. For example, van Winkle and Salthouse (1976) reported the presence of high level of cellular response to silk suture observed from histological study which was confirmed by Salthouse (1982) and Salthouse and Matlaga (1975). Enzyme histochemistry is also useful for studying the biodegradation mechanism of absorbable sutures. 

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