Immunotoxin reductive amination

A second method of immunotoxin preparation by reductive amination involves the use a polysaccharide spacer. Soluble dextran may be oxidized with periodate to form a multifunctional crosslinking polymer. Reaction with antibodies and cytotoxic molecules in the presence of a reducing agent forms multivalent immunotoxin conjugates. The following sections discuss these options. 

Figure 21.15 A periodate-oxidized dextran polymer may be reacted with both an antibody and an intact toxin component using reductive amination to form a multivalent immunotoxin complex.

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