Immunosensors amperometry

In amperometry, the current produced by the oxidation or reduction of an electroactive analyte species at an electrode surface is monitored under controlled potential conditions. The magnitude of the current is then related to the quantity of analyte present. However, as both antibody and antigen are not intrinsically electroactive, a suitable label must be introduced to the immunocomplex to promote an electrochemical reaction at the immunosensors. In this respect, enzyme labels including the... 

Rotating disk amperometry was used to measure these immunosensor responses using H2O2 to activate HRPFe to a ferryloxyHRP form (HRPFe = O), and hydroquinone (HfJ) to mediate the reduction of HRPFe = O (Scheme 1.1). The sensor response is a steady state amperometric current proportional to protein concentration (see Fig. 1.5a). 

---