Immunoreactors with Electrochemical Detection

An immunostirrer for the determination of creatine kinase (CK) isoenyzme MB based on alkylamine glass-immobilized anti-IgG antibody has been proposed by Yuan et al. (1981). By binding of creatine kinase to antibody, only the CK-M subunit but not the CK-B subunit is inhibited. The remaining CK-B activity was measured by electrochemical oxidation of ferrocyanide formed in the following coupled reaction  

With a maximum binding capacity for CK-M of800 U/l and a CV of 4-5% the system was stable for 52 days. 

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