Immunoperoxidase-diaminobenzidine

In the development of the specific antigens, the immunoperoxidase methods are used [147]. At 4°C, the overnight incubation of acetone-fixed frozen sections in the presence of the primary antibody is followed by developing immunoperoxidase with 3,3 -diaminobenzidine tetrahydrochloride. At 4°C, the overnight deparaffination of acetone-fixed frozen sections in the presence of the primary antibody is also followed by developing immunoperoxidase with 3,3 -diaminobenzidine... 

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