Immunolabeling studies

Use the serum for immunolabeling studies without any further purification steps. 

The detailed distribution of polysaccharides within cell walls can be determined by immunolabeling sections of plant tissues with appropriate antibodies (Knox, 2008). Such studies also show the distribution of polysaccharides in the middle lamella (Figure 3.5), which develops from the cell plate, formed at cell division, and is responsible for cell-cell adhesion. Cell comers (tri-cellular junctions) and the comers of the intercellular spaces can be regarded as extensions of the middle lamella. They are where stresses that tend to separate plant cells are concentrated and have been referred to as reinforcing zones (Jarvis et al., 2003). These zones and the middle lamella are rich in pectic polysaccharides, but contain no cellulose microfibrils (Jarvis et al., 2003). 

Rapid. Permanent preparations. Simple nuclear counterstains may be used for studying tissue architecture in relation to immunolabeling. 

Gultekin SH, Dalmau J, Graus Y, Posner JB, Rosenblum MK. Anti-Hu immunolabeling as an index of neuronal differentiation in human brain tumors A study of 112 central neuroepithelial neoplasms. Am J Surg Pathol 1998 22(2) 195-200. 

Colloidal gold probes are the most popular of all the immunolabeling techniques for electron immunocytochemistry. Since individual gold probes ctin be easily identified with the electron microscope, there is increased interest in muldple immunolabeling and quandtative studies. The technique is so popular that there are many different nuances in immunolabeling technique. The methods given in this chapter are those that have proved to be sadsfactory in this laboratory. 

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