Immunolabeling cryosectioning

Slot, J. W. and Geuze, H. J. (1984) Gold markers for single and double immuno-labeling of ultrathin cryosections, in Immunolabelling for Electron Microscopy (Polak, J. M. and Vamdell, I. M., eds.), Elsevier, New York, pp. 129-142. 

Rangell, L. K., and Keller, G.-A. 2000. Application of microwave technology to the processing and immunolabeling of plastic-embedded and cryosections. J. Histochem. Cytochem. 48 1153-1159. 

Y-D. Stierhof, H. Schwarz, M. Durrenberger, W. Villiger, and E. Kellenberger, Yield of Immunolabel Compared to Resin Sections and Thawed Cryosections, in Colloidal Gold Principles, Methods and Applications, Vol. 3 (ed. M. A. Hayat), Academic Press, New York, 1991, pp. 87-95. 

Preparation of thawed cryosections for LM 252 Immunolabelling 0.5 (xm sections for LM 253 Preparation of thawed cryosections for EM immunolabelling 253 Immunolabelling thawed cryosections 254... 

Two fundamentally different approaches to EM immunolabeling are known as preembedding and postembedding labeling. Within the category postembed are two main divisions, depending on whether cryosections or resin sections are used. 

The preparation ofultrathin cryosections (cryoultramicrotomy) for transmission electron microscopy (EM) offers a number of advantages such as highest sensitivity, the possibility of using osmium tetroxide as a counterstain after immunolabeling, and the study of, e.g., the intracellular pathways of neuropeptide secretion [37, 38]. However its high cost and technical difficulties have greatly limited its diffusion. In addition, only very small areas of... 

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