Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Immunohistochemistry avidin—biotin method

The avidin-biotin system was developed for detecting antigens at the electron microscope level (Heitzmann and Richards, 1974). Later Heggeness and Ash (1977) proposed the use of this system for fluorescence immunohistochemistry. Guesdon et al. (1979) proposed a variety of labeled avidin-biotin methods which were further supported by Warnke and Levy (1980). The avidin-biotin methods used today are similar to the system described by Hsu et al. (1981). This system is a significant improvement over the previous immuno-histochemical techniques. The problem of endogenous biotin is discussed on page 98. [Pg.216]

A variety of techniques (Fig. 1), which use a variable combination of antibody steps, enzyme labels, and chromogens, are employed in immunohistochemistry. The most common immunohistochemical methods are the PAP (peroxidase-antiperoxidase), ABC (avidin-biotin) peroxidase or alkaline phosphatase, and the APAAP (alkaline phosphatase-anti-alkaline phosophatase) techniques however,... [Pg.297]


See other pages where Immunohistochemistry avidin—biotin method is mentioned: [Pg.12]    [Pg.15]    [Pg.143]    [Pg.60]    [Pg.73]    [Pg.61]    [Pg.208]    [Pg.263]    [Pg.5]   
See also in sourсe #XX -- [ Pg.61 , Pg.62 ]




SEARCH



Avidin

Avidin-biotin

Immunohistochemistry

© 2024 chempedia.info