Immunoglobulins using

The number of sulfhydryls created on the immunoglobulin using thiolation procedures such as this one is more critical to the yield of conjugated enzyme molecules than the molar excess of maleimide-activated enzyme used in the conjugation reaction. Therefore, it is important to use a sufficient excess of Traut s reagent to obtain a sufficient number of available sulfhydryls. 

Weston, P.D., Devries, J.A., and Wrigglesworth, R. (1980) Conjugation of enzymes to immunoglobulins using dimaleimides. Biochim. Biophys. Acta 612, 40-49. 

A secondary antibody labeled with biotin, which is reactive against the species of immunoglobulin used for the primary reagent. 

The various immunoglobulins used for passive immunization are listed in table 13.1.3. 

Ito S, Imura T, Fukuoka T, Morita T, Sakai H, Abe M, Kitamoto D (2007) Kinetic studies on the interactions between glycolipid biosurfactant-assembled monolayers and various classes of immunoglobulins using surface plasmon resonance. Colloids Surf Biointerfaces 58 165-171... 

Kruger, N. J. and Hammond, J B. W. (1988) Purification of immunoglobulins using protein A-Sepharose, in Methods in Molecular Biology, vol 3 New Protein Techniques (fNsAVsx, I M., ed), Humana, Clifton, NJ, pp 363-371... 

Table 23.4 Immunoglobulins used in the prevention and treatment of infections in humans...

An antibody is a protein (one of the immunoglobulins) used by the immune system of a mammal to identify and neutralize antigens (foreign objects like bacteria, viruses and others). Each antibody recognizes a specific... 

Precipitation of the antigen bound to antibody by using a second antibody. If, for example, the primary antibody is obtained from sheep, the second antibody can be antiserum raised against sheep immunoglobulins using a different animal such as a horse. 

Muramatsu H., Tamiya E. and Karube I. (1989) Detomination of microbes and immunoglobulins using a piezoelectric biosensor. J. Membrane Sci., 41. 281-290. 

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