Immunoglobulin papain treatment

Figure 33.3. Immunoglobulin G Cleavage. Treatment of intact IgG molecules with the protease papain results in the formation of three large fragments two Fj,(, fragments that retain antigen-binding capability and one fragment that does not.

The 7 S antibodies of the dogfish shark and of the lamprey are almost unaffected by treatment with papain in neutral buffer (29). Klapper et al. investigated the effects of papain and trypsin on the 19 S and 7 S immunoglobulins of the lemon shark, and on 7 S molecules obtained by... 

---