Immunofluorescence micrographs

Although some report that antibodies are able to fully penetrate cell walls, immunofluorescence micrographs of cell-wall protein localizations suggest that this is not the case (23). Antibody solutions infiltrated under vacuum into stem... 

Figure 14. Immunofluorescence micrograph of microtubule orientation during cell regeneration in Boergesenia forbesii 1.5 h after wounding.

Figure 20. Immunofluorescence micrograph of microtubule orientation in 8 day old cells of Boergesenia forbesii. Highly ordered microtubules are oriented longitudinally to the cell axis (double-headed arrow).

Figure 21. Immunofluorescence micrograph of perinuclear microtubules in the aplanospore of 3h post-wounding of Valonia ventricosa.

Figure 23. Immunofluorescence micrographs in the aplanospore of 3h post-wounding of Boergesenia forbesii. Figure 23a is focused on the perinuclear microtubules, while Figure 23b is focused on the cortical microtubules which are oriented randomly.

Immunofluorescence micrographs demonstrating the effect of 1. Oumol/L paclitaxel on the distribution of the contractile filament smooth muscle a-actin and the intermediate filament vimentin in haSMCs. 

Although some report that antibodies are able to penetrate cell walls fully, immunofluorescence micrographs of cell-wall protein localizations suggest that this is not the case (21). Antibody solutions infiltrated under vacuum into stem tissues do not appear to reach inner surfaces of the cell walls, whereas antibodies applied to cut surfaces of the stem clearly do (see Fig. 3 in ref 21). In order to facilitate the penetration of antibodies into plant cells, the cell walls need either to be opened or removed. This is most often accomplished by digestion with the enzymes cellulase and/or pectinase (22). 

Fig. 7. An electric field induces cathodal distribution of EGF receptor and activation of ERK. Immunofluorescence micrographs show the asymmetry of (a) EGER and (b) dual-phosphorylated ERK1/2. Cells were exposed to an electric field of 150 mV/mm for 1.5 h. (Modified from...

Figure 7-32 Micrograph of a mouse embryo fibroblast was obtained using indirect immunofluorescence techniques.313 The cells were fixed with formaldehyde, dehydrated, and treated with antibodies (formed in a rabbit) to microtubule protein. The cells were then treated with fluorescent goat antibodies to rabbit /-globulins (see Chapter 31) and the photograph was taken by fluorescent light emission. Courtesy of Klaus Weber.

Micrographs of the same field (A), phase-KJontrast (B), immunofluorescence double staining with ceil type specific marker galactocerebroside (GC) and (C), with a monoclonal antibody against NT3R. Bar corresponds to 7 pm ... 

Fig. 7. Fluorescence micrograph of a reovirus type 3-infected CV-1 cell demonstrating vimentin filament organization within viral inclusions. Cell was subjected to indirect immunofluorescence microscopy using antibody against vimentin (bar = 20 p,m). From Sharpe et al. (1982), by permission of Virology.

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