Immunocytochemistry multiple markers

Until 1980, peroxidase was the marker of choice, but since then, the use of colloidal gold has increased and now, is almost universally used for electron immunocytochemistry Colloidal gold is ideal for electron microscopy. It is particulate tind very dense, therefore, it can be identified on heavily stained biological tissue. Because it is small, it will not obscure the fine structure of the sample, and it can be prepared in several different sizes to be used for multiple immunolabeling experiments and quantification. 

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