Immunoblotting membrane matrices

Nitrocellulose and polyvinylidene difluoride (PVDE) are the types of membrane matrix most commonly used in immunoblotting. I mterol 5 uses nitrocellulose membrane, which has high binding capacity for proteins (approx. 

Nevertheless, planar electrophoretic techniques are very popular in biotechnological and biochemical laboratories. There is still no online interface, directly linking such techniques with mass spectrometric detectors. A handful of experiments were described, regarding the identification of proteins transferred onto the membrane after immunoblotting [6,7]. Secondary ion mass spectrometry (SIMS) and matrix-assisted laser desorption/ionization (MALDI) techniques were used for the direct analysis of intact proteins or after sputtering blots with trypsin solution for digestion... 

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