Immobilization techniques CLEAs

For an assessment of different immobilization techniques, CLEAs of HbHNL, PaHNL, and MeHNL were compared with aqua gels of these enzymes [68]. When the CLEAs were used in the presence of a buffer layer that is under conditions comparable to the aqua gels (see also Section 2.3), aU of them were stable [68, 80] and PaHNL can be recycled up to 10 times without loss of activity [81]. The real advantage of the immobiUzation as CLEAs only becomes obvious when they are employed in pure organic solvents. Here MeHNL displays remarkable stability... 

Enzyme immobilization techniques have been revealed as a powerful tool to enhance most enzyme properties such as sta-bihty, activity, specificity and selectivity, and reduction of inhibition. Several methods had been used including cross-linked enzyme aggregates (CLEAs) (Wang et al. 2011), entrapment, and support-based immobilization (Brady and Jordaan 2009). 

Our group has developed a technique that forms an enzyme-immobilizing membrane on the microcharmel surface [154]. This is a modification of CLEA formation, which is used in batchwise organic synthesis [155]. The microreactor has a cylindrical membrane composed of cross-linked polymerized acylase and polylysine matrix product on the internal surface of the microchatmel (Figure 10.28). 

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