Immobilization magnetic supports

Coupling proteins (by covalent methods) to magnetic supports is the most common approach (105). Various classes of proteins, such as lectins (110) and antibodies (111), and a large number of enzymes including invertase (112,113), papain (106, 114) a-chymotrypsin (114, 115), P-galactosidase (114), adenylate kinase (112), acetate kinase (112), and horseradish peroxidase (112), have been immobilized. In fact, such supports are now commercially available, for example, Enzacryl FEO-M (available from Aldrich Chemictd Company, USA) and Dyna (sold by Dynal, Norway). 

Stability and stabilization of enzymes. Elsevier, Amsterdam, pp 111-131 Munro PA, Dunnill P, LiUy MD (1981) Casein hydrolysis in a stirred tank reactor using chy-motrypsin immobilized on magnetic supports. Biotechnol Bioeng 17(10) 1515-1528 Naik S, Karanth N (1978) Effect of internal and external diffusion on the apparent stabiUty of immobUised enzyme catalysts. J Appl Chem Biotechnol 28 569-580 O Fagain C (2003) Enzyme stabilization - recent experimental progress. Enzyme Microb Technol 33 137 149... 

A short review on the preparation and uses of immobilized microbial cells has tabulated information that has appeared in the literature. The potential use of magnetic supports in separations of biologically active proteins has been discussed. ... 

Selected signature libraries may be immobilized on a solid matrix such as activated silica resin, cellulose microporous modified membranes [66], Sepharose , magnetic beads based on MagaPhase technology. The affinity support obtained is used for IgM antibodies parting. 

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