Immobilization carbonyldiimidazole activation

Immobilized soybean lipoxygenase With carbonyldiimidazole-activated matrix termed Reacti-Gel(g) 13-Hydroperoxy-acid from linoleic acid Octane-borate buffer pH9 (5/2) No surfactant is required 24... 

The density of immobilized proteins was successfully improved when a dendrimer-activated surface was used.24 Poly(propyleneimine) dendrimers were bound to the Si and glass surface that was modified with l,l -carbonyldiimidazole under anhydrous conditions. Due to the high concentration of charged amine groups of dendrimers, this method provides a fine platform for high density protein immobilization. 

Fig. 6 Immobilized functional nucleic acids, a Example fabrication of an aptamer biosen- sor surface (i) Attachment of (glycidoxypropyl)trimethoxy silane (GOPS) to glass surface at low pH results in the formation and subsequent cleavage of an epoxide, (ii) The resultant diol is activated with 1,1-carbonyldiimidazole (CDI) to form a carbonylimidazole.

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