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Immobilised metal affinity chromatography IMAC

Figure 2.26 Immobilised Metal Affinity Chromatography (IMAC). Metal ions (for instance, Ni + ions) are gel-immobilized by chelation to groups covalently attached to the solid support. Histidine (His) residues in proteins (or part of a His-tag) have a high affinity for the immobilized ion. Elution is typically carried out by using a competitively high concentration of imidazole. Figure 2.26 Immobilised Metal Affinity Chromatography (IMAC). Metal ions (for instance, Ni + ions) are gel-immobilized by chelation to groups covalently attached to the solid support. Histidine (His) residues in proteins (or part of a His-tag) have a high affinity for the immobilized ion. Elution is typically carried out by using a competitively high concentration of imidazole.
Gels maybe eliminated by shotgun proteomics, but specihc protein modihcations of interest need to reviewed and resolved by some form of chromatography prior to mass spectrometry. For example, phosphopeptides maybe specihcally purihed pre mass spectrometry using immobilised-metal affinity chromatography (IMAC) (see Chapter 2). Both iron (as Fe +) and... [Pg.508]

Another very important form of affinity chromatography is immobilised metal ion affinity chromatography (IMAC) (Kagedal 1989 Yip and Hutchens 1996). This... [Pg.95]

See other pages where Immobilised metal affinity chromatography IMAC is mentioned: [Pg.403]    [Pg.131]    [Pg.411]    [Pg.403]    [Pg.131]    [Pg.411]   


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