Immersion matrix application method

Several methods have been used for the matrix application (1) immersing a tissue section quickly in a matrix solution, (2) spraying matrix solution onto a tissue section with an air brush, (3) putting small droplets of matrix solution onto a tissue section with an automatic pipetting device that can dispense picoliter volumes (lpL = 10 pk) of reagents.4,5,715161819... 

As discussed earlier, it is important that corrosion inhibitors are transportable through the resin matrix and therefore this transport was investigated using radiochemical, spectroscopic and conductimetric methods on dry and water immersed resin, different detection techniques being applicable to different inhibitor species. In all cases it was found that diffusion was negligible in dry samples but that diffusing water could "pick up inhibitor species and transport them sufficiently well to compensate for any small amount of depletion of inhibitor at the chip surface. 

Trimmed and faced blocks are stained by immersion in a 10% solution of HgO in trifluor-oacetic acid for 10-60 min. Samples are washed in a dilute solution of trifluoroacetic acid followed by distilled water. Poly(phenylene oxide) (PPO) appears to have a higher mercury uptake than polystyrene in a bonded laminate of the two films [6]. Blends made by coextrusion of Kraton G and PPO show dispersed particles in a matrix. Kraton G is the lighter contrast polymer as it seems to take up less of the stain than the PPO. In summary, mercuric trifluoroacetate staining has been shown for several polymers where the dispersed phase particles are differentiated by this stain. The method has limited application. 

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