Imaginal disc dissection

Larval Eye Imaginal Discs ), but instead of dissecting the eye-antenna discs and attached structures dissect only the salivary glands (Fig. la, b). 

Add glycerol PBS (1 1) to the tube and immediately remove the samples with a cut tip (while they are floating) and place onto a slide. Dissect out the optic lobe with needles and mount the slide with only the eye disc, or retina, on it (see Note 21). Alternatively the slides with imaginal discs can be mounted as previously described for embryos (see Staining and Mounting on Slides ). The samples can be circled with a nonfluorescent pen to facilitate viewing. 

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