Identification of a BFD Variant with an Optimized Acceptor Aldehyde Spectrum

4 Identification of a BFD Variant with an Optimized Acceptor Aldehyde Spectrum 

The BFD mutant library generated by epPCR was expressed in microtiter plates and 8000 clones were subjected to the carboligation assay with benzaldehyde 4 and dimethoxyacetaldehyde 8 as the substrates. The reaction was incubated for 24 h at 30 °C as suggested from experiments using the positive control strain 

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