Hypothyroidism, neonatal immunoassay

Table 10 gives a list of enzyme immunoassays of substances related to neonatal hypothyroid screening. The methods for measuring substances in dried blood can be used for mass screening and those for tests on serum can be used for babies who are recalled. 

In 1976, we developed a double-antibody enzyme immunoassay of TSH using alkaline phosphatase, but this method was not very sensitive (M8). As shown in Table 10, several other methods were later reported (A3, 12, K2, K3, M7) and two methods have been used to measure TSH in dried blood samples in screening for neonatal hypothyroidism in Japan. 

Enzyme Immunoassay for Substances Related to Neonatal Hypothyroid Screening... 

Free Thyroxine. Measurement of free T4 radioimmunoassay in dried blood samples has been found to be useful to avoid false-positive results for low TBG in neonatal hypothyroid screening. Enzyme immunoassay of free T4 in serum was developed by Weetall et al. (W7) and subsequently by us (16). 

M13. Miyai, K., Tsuruhara, T., Kusuda, S., Ishibashi, K., Kawashima, M., Mizuta, H., Nose, O., Yabuuchi, H., and Oura, T., Semiautomated enzyme immunoassay of thyrotropin as a mass screening test for neonatal hypothyroidism. Pediatr. Res., 18, 1289-1292 (1984). 

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