Hypersil Advance

Overnight, six polar columns should be inserted into the column selecting valve (e.g., from very polar, like Platinum EPS or Hypersil ADVANCE, to moderately polar, like liChrospher or XTerra). 

Fluofix INW Fluofix lEW Platinum EPS Hypersil ADVANCE SynergiPOLAR RP... 

Reagents were obtained from commercial sources and used as received. V-Boc anthranilic acid 13 was purchased from Advanced Chem-Tech and V-Boc-methyl anthranilic acid 14 was purchased from Aldrich. Proton nuclear magnetic resonance ( H NMR) spectra were run at 500 MHz. LC/MS analysis was performed using a Ci8 Hypersil BDS 3-prn 2.1 x 50-mm column (UV 220 nm) with a mobile phase of 0.1% TFA in CH3CN/H20, gradient from 10% CH3CN to 100% over 5 or 15 min and APcI ionization. 

---