Hydroxyproline peroxide

The hydrox)dation system consists of bivalent iron complex bound by ethylenediamine tetraacetic acid (formula [187]), ascorbic acid (formula [53]), and hydrogen peroxide (Brodie et al. 1954, Udenfriend et al. 1954). In vitro, the major amount of hydroxyproline was formed during the first 30 s (Hurych 1967). If the proline in the incubation mixture was replaced by hydroxyproline a decomposition of about 30 % of the hydroxyproline occurred. Accordingly, the newly formed hydroxyproline was the result of synthetic and degradation processes, which are caused, as is generally assumed (Haber and Weiss 1934, Kolthoff and Medalia 1949), by free radicals. 

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