Hydroxyproline arabinosides

Akiyama, Y. and K. Kato Structure of hydroxyproline-arabinoside from tobacco cells Agr. Biol. Chem. 41 (1977) 79-81. 

Akiyama, Y., Mori, M. Kato, K. (1980) C-NMR Analysis of Hydroxyproline Arabinosides from Nicotiana tabacum Agricultural and Botanical Chemistry, 44, 2487-489... 

Methylation analysis of the tetra-arabinosides isolated from primary cell walls of tomato and sycamore indicates that the arabinosyl residues are terminal and 2- and 3-linked (71, 123). Akiyama and Kato (2) have studied the hydroxyproline arabinosides obtained from suspension-cultured cells of tobacco (Nicotiana tabacum). These workers used periodate oxidation (Smith degradation), methylation analyses, NMR, and optical rotation to show that the structure of the hydroxyproline tetra-arabinoside is Araf-(1 3)-Araf-(l - 2)-Arar(l - 2)-Arar(l - 4)-hydroxyproline. 

Single galactosyl residues are glycosidically attached to the serine hydroxyls of the hydroxyproline-rich cell wall proteins of suspension-cultured tomato cells (84, 85). This was shown by removing the arabinosides from the intact cell walls by acid hydrolysis (pH 1 for 1 hr at 100° C). The hydroxyproline-rich wall protein, with arabinosyl residues removed, is susceptible to proteolysis with trypsin. The resulting solubilized tryptides have been separated by cation exchange and gel filtration chromatography. The composition of the tryptides has been determined by amino acid analysis (85). Some of the tryptides have been sequenced by subtractive N-terminus identification and further partial acid hydrolysis (85). 

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