4"-Hydroxyl group, chemical modification before

As mentioned before, the chemical modification of peroxidases is based on the reactivity of their exposed amino acids, besides their prosthetic group, ferroproto-porphyrin IX. As peroxidases are glycosylated enzymes, the hydroxyl groups of the carbohydrates can be modified as well (Fig. 9.1). 

Hence, the technique we developed allowed us to perform a SCR in the region scanned by a probe microscope and to plot maps of the adhesion forces for the AFM probe to the same pol5mier surface area before and after its modification by the selective reagent. As a result, we have obtained information on the local chemical stmcture of the studied plasma-polymerized coating with an area resolution no worse than 36 nm. It has been established that hydroxyl groups on the studied surface are positioned mainly along topographic folds on the film surface [28],... 

---