Hydrolytic Release of Sialic Acids from Sialobiopolymers

Hydrolytic Release of Sialic Acids from Sialobiopolymers 

Preparation and Analysis of Pertrimethylsilylated N,0-Acylneuraminic Acids (Casals-Stenzel et al. 1975) 

Sialic acid dried over P2O5 (10-lOOp.g) is mixed with N-trimethylsilylimidazole (25(ji.l) in a test tube. The tube is flushed with nitrogen and whirled for either 

Analysis of Acetyl andlor Glycolyl Residues of Sialic Acid (SuGiTA 1979 a) 

Glycoconjugate dried over P2O5 (1 mg) is treated with l.ON n-butanolic HCl for 3 h at 100 °C. After cooling, the acidic -butanol solution is neutralized with silver carbonate (pH-paper). An aliquot of the n-butanol solution is analyzed for n-butyl acetate and/or n-butyl glycolate by g.l.c. on 3% diethylene glycol succinate on Shimalite, 80-100 mesh, at an oven temperature of 40°C (column 2m x 3mm, i.d.). 

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