Hydrolysis testicular-type

Figure 2. Hydrolysis of the P(1,4) glycosidic bonds of HA as catalyzed by testicular-type HAases (EC 3.2.1.35) (Deschrevel, in press).

The discovery, ca. 1968, by B. Rosenberg, that the cis isomer has anti-tumor activity stimulated the synthesis and screening of over 2000 different types of complexes with different amines and anionic ligands. Platinum compounds are among the most effective medications for the treatment of advanced cancer PtCl2(NH3)2 ( Cisplatin ) is mainly used for the treatment of testicular and ovarian cancer. One of its drawbacks are the severe toxic side-effects which may be related to the facile hydrolysis of Cl ligands under physiological conditions ... 

Cisplatin has been extensively used for the treatment of testicular and ovarian cancers and increasingly against other types of solid tumors (head/neck, lung, cervical, and bladder) [53]. The mechanism of action is believed to involve its aquation to the more reactive aqua-chloro complex, c/x-[PtCl(NH3)2(OH2)]+ prior to binding to DNA in vivo [50,51,54-56]. Therefore, it is important to determinate the rates of hydrolysis and pKa values of the hydrolysis products. 

Type 2. Hyaluronidase from leech saliva. The final products of hydrolysis by both Type 1 and Type 2 hyaluronidases are tetrasaccharides, which possess amino sugar at their reducing terminus and are activated with the enzymes of the Type 1. Additionally, the testicular and lyzosomal hyaluronidases are capable of trans-glycosylase activity and... 

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