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Hydrolysis of p-Nitrophenyl--D-Galactoside with -Galactosidase from E. coli

Hydrolysis of p-Nitrophenyl-/ -D-Galactoside with / -Galactosidase from E coli [Pg.34]

The purpose of the present experiments is to characterize kinetics parameters of p-nitrophenyl-/3-D-galactoside hydrolysis with / -galactosidase. Students will find out how to use absorption spectroscopy to study enzymatic properties of an enzyme (see also Murata et al. 2003). Before entering the lab, students should be able to explain the Beer-Lambert-Bouguer law and the basics of enzymology. [Pg.34]



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D-Galactosidases

D-Galactoside

E. coli

Galactosidase

Galactosidases from E. coli

Galactosidasic

Galactoside

Galactosides

Hydrolysis nitrophenyl

Of p-galactosidase

P hydrolysis

P-D-Galactosidase

P-D-Galactosidases

P-Galactosidase

P-Galactosidases

P-Galactosides

P-Nitrophenyl galactosides

P-galactoside

P-nitrophenyl

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