Hydrogen peroxide Term Links

The terms NEFA, unesterified fatty acids, and free fatty acids have been used synonymously in the literature, although these fatty acids are mainly bound to plasma proteins. Enzymatic assays are convenient with NEFA in the presence of added acyl-coenzyme A (CoA) synthetase forming acyl CoA esters, which are then oxidized by acyl CoA oxidase to yield hydrogen peroxide. The hydrogen peroxide produced is measured by a peroxidase-linked colorimetric detection reaction. Plasma samples should be separated promptly and stored frozen to prevent in vitro lipolysis (Zambon, Hashimoto, and Brunzell 1993). 

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