Hydrogen Dipyridamole

Urea maybe reacted with acetoacetic ester and that product nitrated to give 5-nitro-orotec acid That is hydrogenated, then reacted with urea and potassium cyanate to give tetrahydroxypy-imidopyrimidine. The tetrahydroxy compound Is converted to the tetrachloro compound POCI3. Reaction with diethanolamine and then with piperidine gives dipyridamole. 

Nakushima et al. determined dipyridamole on the basis of its chemiluminescence intensity after injecting into a carrier stream of triethylamine in acetonitrile (1 mL/min) combined with a stream of bis-(2,4,6-trichlorophenyl)oxalate and hydrogen peroxide [47]. 

Zhou et al. developed an HPLC method for the determination of dipyridamole, aspirin, and salicylic acid [67]. The HPLC system consisted of a Lichrospher 5-Cig (4.6 mm x 150 mm) column and a UV detector operated at 227 nm. The mobile phase (eluted at a flow rate of 1 mL/min) was composed of disodium hydrogen phosphate solution(methanol (50 50, pH adjusted to 3 by addition of phosphoric acid). The mean recovery of dipyridamole and aspirin was 100.9-104.1% and 97.01-97.89, respectively. 

IL-10 production after hydrogen peroxide and IPS stimulation and dose-dependently inhibited TNF secretion. The enhanced IL-10 production was not observed when cells were preincubated with adenosine Ai or Aj receptor agonists (E-phenylisopro-pyladenosine, 5 -N-ethylcarboxamide-adenosine, and 2-chloroadenosine) and was not affected by pre-treatment with theophylline, an antagonist of both Ai and Aj receptors, or with dipyridamole, an inhibitor of adenosine cellular uptake (Le Moine et al. 1996). 

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