Hydrogels-based cell encapsulation limitations

As for animal cell entrapment in hydrogel microparticles or microcapsules, encapsulation procedure should proceed under physiological conditions within a short time (20-30 min), in order to provide cell viability, and to be as simple as possible because all manipulations are carried out under strictly sterile conditions. Taking into account all these requirements, it should be noted that the list of polymer materials and methods for animal cell encapsulation is rather limited. So-called alginate-based carriers (microparticles, micro- and nanocapsules) assure the favorable polymer systems for animal cell immobilization. 

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