Hydrocyanation and Hydrolysis in a Bienzymatic Cascade

Stabilization oT NLases as Cross-linked Enzyme A regates 

We conclude from the formation of nearly enantiomerically pure (S)-3a that the bienzymatic methodology is basically sound and that racemisation of the interme- 

Furthermore, we considered that the hydrocyanation of cinnamic aldehyde (2c) suffers from an unfavorable equilibrium [7, 8] and (apparent) mediocre enantioselectivity [7], which could be increased to 96% only upon careful optimization [8]. We reasoned that the equilibrium would be improved by removing the cyanohydrin Ic by in-situ hydrolysis. Moreover, such a bienzymatic procedure would also obviate the erosion of the cyanohydrin ee which tends to become apparent at the 

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