Hydrazide covalent conjugation

Figure 8.3. Examples of small molecules employed In site-specific labeling methods. (A) Structures of FLAsH and a 4 -phosphopantetheinyl-fluorophore conjugate. (B) Structure of a BG-fluorophore conjugate and a scheme for the covalent attachment of the fluorophore to AGT. (C) Structures of biotin and the ketone analogue of biotin. Attachment of the ketone analogue to an AP-protein fusion and subsequent reaction with a fluorophore hydrazide for covalent fluorophore attachment.

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