Hydration freeze-dried lysozyme

Lysozyme from chicken egg white (Wako) was used without further purification. In the experiment evaluating the influence of the IR spectra of lysozyme, all solutions or moisture were prepared in D2O. A quantity of 20 mg of lysozyme and 100 mg of trehalose was dissolved in 1 ml D2O. The freeze-drying sequence and adjustment of residual water was carried out according to the method described above. The following four samples were prepared 200mg/ml lysozyme D2O solution (hydrate LYS), fully freeze-dried lysozyme containing trehalose (dry LYS/TRE), freeze-dried lysozyme containing trehalose and 5.5% residual water (moist LYS/TRE), and fully freeze-dried lysozyme without trehalose (dry LYS). 

The Effect of Trehalose and Residual Water on the Degree of the Hydration Structure of Freeze-Dried Lysozyme... 

Remmele et al. [3.55] studied with infrared spectroscopy the structure-hydration behavior of a 49.4 mg/mL lysozyme D20 solution with and without 10 % sucrose. The sample was cooled n in the measuring chamber to -100 °C and then connected to a freeze drying installation, after which the temperature of the sample was raised to +40 °C ... 

---