Hydralazine, HPLC

Bulk Hydralazine HPLC Dilute with pH 7 buffer Agarose pH 7.0 buffer 280 nm EP, pt. 11-8, p. 338-4 [6]... 

V. Das Gupta, Quantitation of hydralazine HC1 in pharmaceutical dosage forms using HPLC, J. Liquid Chromatogr., 5 2497 (1985). 

Lessen T. ao D-C. Interactions between drug substances and excipients. 1. fluorescence and HPLC studies of triazolr thalazine derivatives from hydralazine hydrochloride and starch. J Pharm Sci 1996 85 326-9. 

Chemical reactivity is another way of testing the purity of a drug substance. Hydralazine hydrochloride is part of a class of compounds called hydrazine derivatives. It is well known that hydrazine derivatives react with aldehydes and ketones to give hydrazones.The test is conducted by taking a standard reaction of hydrazine with benzaldehyde and comparing it to the same reaction of hydralazine with benzaldehyde as in Scheme 4. By using an HPLC procedure, standard solutions of each derivative are injected, and by comparison of the peak responses, the amount of hydrazine is determined. 

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