Hydra oligactis

Herring CO, Adams JA, Wilson BA, et al. 1988. Dose-response studies using ethylene dibromide (EDB) in Hydra oligactis. Bull Envir Contam Toxicol 40 35-40. 

Fig. 1. Hydra oligactis whole mount labeled with monoclonal antibody JDl. (A) Isolated ganglionic neuron in the body column, scale bar = 50 om (B) hypostomal nerve net with sensory neurons of the mouth at the left and ganglionic neurons of the perihypostomal ring to the right, scale bar = 50 pm (C) cell bodies of hypostomal sensory neurons extending from the mesoglea (processes) to the surface of the ectoderm, scale bar = 25 pm.

Fig. 2. Hypostome (mouth) of a Hydra oligactis whole mount labeled with monoclonal antibody DB5. (A) Nonconfocal image of sensory neurons and processes (B) confocal optical section of the same field as (A) illustrating details of neuronal cell bodies and processes. Scale bar = 25 pm.

Fig. 3. Head of a Hydra oligactis labeled with monoclonal antibody DBS. (A) Neurons of hypostome and perihypostomal ring (left) and base of tentacle (right) at high magnification (x 20 objective) note considerable out-of-focus labeling of the tentacle (B) same specimen as in (A) but at lower magnification (x 10 objective) although the hypostome is out of focus, the neuronal net of the tentacles is much clearer. Scale bars = 50 pm.

Fix the specimen. For this example, the live coelenterate Hydra oligactis is first relaxed using an anesthetic solution of 2% urethane in hydra culture medium for 1 min to prevent contraction into a ball on fixation (17). 0.5 mL is used in a well of a 24-well tissue-culture plate. 

Javois, L. C. and Tombe, V. K. (1990) Head activator does not qualitatively alter head morphology in regenerates of Hydra oligactis. Roux s Arch. Dev. Biol. 199, 402 08. 

Bosch, T.C.G., Krylow, S.M., Bode, H.R. Steele, R.E. (1988). Thermotolerance and synthesis of heat shock proteins these responses are present in Hydra attenuata but absent in Hydra oligactis. Proceedings of the National Academy of Sciences (USA) 85, 7927-31. 

Fix the specimen. For this example, the live coelenterate Hydra oligactis is first relaxed using an anesthetic solution of 2% urethane m hydra culture... 

Octopus vulgaris (Mollusca), Physalia sp.. Hydra oligactis. Hydra littoralis (Coelenterata), and Sycon (or Scypha) ciliatum (Porifera) reference 122,243,277. ... 

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