Hyaluronic acid solution

B2. Balazs, E. A., and Sundblad, L., Viscosity of hyaluronic acid solutions containing proteins. Acta Soc. Med. Upsalien. 64, 137-146 (1959). 

S. C. De Smedt, A. Lauwers, J. Demeester, Y. Engelborghs, G. De Mey, and M. Du. Structural information on hyaluronic acid solutions as studied by probe diffusion experiments. Macromolecules 27 141-146 (1993). 

Burns JW, Skinner K, Colt J, Sheidhn A, Bronson R, Yaacobi Y, Goldberg EP. Prevention of tissue injury and postsurgical adhesions by precoating tissues with hyaluronic acid solutions. J Surg Res 1995 59 644 52. 

Fig. 117. During its aseptic production the hyaluronic acid solution can be sterilised through a filter (0.22 pm) before the first step of the production process or at the last step (filling). In terminal sterilisation the already filled product is sterilised (e.g., autoclavation)...

S.E. Moulton, M. Maugey, P. Poulin, G.G. Wallace, Liquid crystal behavior of single-walled carbon nanotubes dispersed in biological hyaluronic acid solutions. J. Am. Chem. Soc. 129, 9452-9457 (2007)... 

Mix with sodium hyaluronic acid with NaOH to produce hyaluronic acid solution. It is deaerated to make it a homogeneous solution (excluding the foam). Add the crosslinking agent with cold water, mix well, and let it react for 5 to 6 h at 35 0°C. 

T. C. Laurent, I. Bjork, A. Pietruszkiewicz, and H. Persson. On the interaction between polysaccharides and other macromolecules II. The transport of globular particles through hyaluronic acid solutions. Biochimica et Biophysica Acta, 78 (1963), 351-359. 

De Smedt, etal. used FRAP to examine diffusion of fluorescein-labeled dextrans and polystyrene latex spheres through hyaluronic acid solutions(17). Dextrans had molecular weights 71, 148, and 487 kDa. The hyaluronic acid had and of 390 and 680 kDa. The dextran diffusion coefficients depend on matrix polymer c as stretched exponentials in c, as seen in Figure 9.9b. Hyaluronic acid solutions are somewhat more effective at retarding the larger dextran probes. Viscosities for these solutions were reported by De Smedt, et a/. (18). The concentration dependence of rj is stronger than the concentration dependence of Dp of the polystyrene spheres, which is in turn stronger than the concentration dependence of Dp of the dextrans. Spheres and dextrans both diffuse more rapidly than expected from the solution viscosity and the Stokes-Einstein equation. 

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