Human Serum Albumin with Laser Diode Excitation

Measurement of Human Serum Albumin with Laser Diode Excitation 

While very weak intrinsic emission has been observed from unlabeled DNA, this emission is too weak and too deep in the UV for xactical applications. Fortunately, there we numerous probes whidi spcmtaneously t nd to DNA and display enhanced emission. Several r ie sentative DNA probes are shown in Bguie 3.21. One of the most widely used dyes is eUudium bromide (EB). EB is weakly fluorescent in water, and its iitfensity increases about 30-fbld upon binding to DNA. Ute lifetime of EB is about 1.7 ns in water and increases to about 20 ns upon 

In recent years, improved DNA dyes which bind to l A with high affinity have been developed Typical hi -affin- 

In solution, 2-AP has a high quantum yield and a single exponential decay time near 10 ns. Upon incorporation into DNA double-strand oligomers, its fluorescence is partially quenched and its decay becomes complex. The sensitivity of 2-AP to its environmoit makes It a usefiil probe for studies of DNA cooftMination and dy namics.  

Like 2-AP, DCP Is partially qooidied die in douUe-hdical roiA (Hguie 3.23) but is mac fiuorescmt when 

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