Human alcohol dehydrogenase isozymes

Walsh JS, Reese MJ, Thurmond LM. The metabolic activation of abacavir by human liver cytosol and expressed human alcohol dehydrogenase isozymes. Chem Biol Interact 2002 142(1-2) 135-154. 

Retrieve DNA encoding human alcohol dehydrogenase isozymes in fasta format and perform multiple alignment with ClustalW. 

Retrieve amino acid sequences (in fasta format) of human alcohol dehydrogenase isozymes and perform multiple alignment with ClustalW to evaluate their homology. Identify the amino acid substitutions among the seven isozymes. 

Smith M, Hopkinson DA, Harris H. Alcohol dehydrogenase isozymes in adult human stomach and liver evidence for activity of the ADH3 locus. Ann Hum Genet (London) 1972 35 243-253. 

Retrieve complete CDS of human alcohol dehydrogenase (ADH) isozymes from UniGene and record the comparison with other organisms (UniGene listings). 

The multiplicity of isozyme patterns of alcohol dehydrogenase in human liver (Section II,A,2), together with racial and acquired differences in ethanol metabolism (31,32), have attracted great interest. No clear correlation has, however, so far been found between patterns of alcohol dehydrogenase isozymes and ethanol metabolism (33) or alcoholism. 

The discovery of the steroid-active isozymes of horse liver alcohol dehydrogenase (Sections II,A,l,a and II,A,l,d) also established that 3-keto-and 3 3-hydroxysteroids are substrates, but the functional significance of this is not clear. Since this activity also seems to be present in the rat enzyme (9) and in all isozymes of the human enzyme (W), it may be more important than previously realized when the horse ethanol-active isozyme was the most studied form. It may also be noted that mammalian livers contain many different specific steroid dehydrogenases (11). Structural studies have established that mammalian alcohol dehydrogenases have a distant evolutionary link to both the yeast (ff) and bacterial enzymes (IS). 

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