HPLC system typical instrument components

A system is typically comprised of multiple instrument components. Therefore, there is usually an individual IQ for each of these instruments and for any corresponding instrument control/data-handling software. The typical instrument components making up an HPLC system include a binary or quaternary HPLC pump, an autosampler supporting multiple vials or microtiter plates (autosamplers often include cooled Peltier trays for sample stability), a column oven, and a UV-Vis or photodiode array (PDA) detector. 

A single capillary scale reverse phase high-performance liquid chromatography system coupled online to automated electrospray ion trap tandem mass spectrometry (ID-LCMS) is usually performed to characterize the protein fractions. The following protocol is an example of the implementation of the method to identify the peptide components of tryptic digests of the yeast HPLC fractions analysis using the ion trap tandem mass spectrometer instrument described in Section 13.2.2.5, and typical results are shown in Figure 13.2-3. 

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