Horseradish peroxidase aromatic hydroxylation

Peroxidases have also been utilized for preparative-scale oxidations of aromatic hydrocarbons. Procedures have been optimized for hydroxylation of l-tyrosine, D-(-)-p-hydroxyphenylglycine, and L-phenylalanine by oxygen, di-hydroxyfumaric acid, and horseradish peroxidase (89). Lactoperoxidase from bovine milk and yeast cytochrome c peroxidase will also catalyze such hydroxylation reactions (89). 

The nuclear hydroxylation of aromatics can be catalyzed by horseradish peroxidase, and with suitably activated substrates synthetically useful yields can be obtained.The reactions are usually carried out at 0 C in the presence of dihydroxyfumaric acid cofactor and a source of oxygen. Thus l-DOPA (102) has been prepared using this system. ... 

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